Increasing Gram stain “yield”
Often with isolates that have scanty organisms, the yield from the Gram stain can be low. In the exam, you may be asked how you could increase the yield of the Gram stain. The answer is to perform the layered technique.
This can be done as follows:
- Take one drop of your specimen. Place on the glass slide and allow to dry.
- Repeat the procedure twice more, to complete three layers.
- Gram stain as usual.
The process can be performed for all isolates from sterile sites, but in particular for neonatal CSFs.
AMH.
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